Influenza Virus Production in MDCK Cells or Chicken Eggs

Clients have been coming to VIRAPUR because of our extensive knowledge and experience in influenza virus production, purification and testing. We offer a wide variety of influenza services.

• Propagate influenza in MDCK cells and embryonated Specific Pathogen Free chicken eggs to produce high titer virus in media or allantoic fluid. We are a premier producer of influenza virus in injected eggs.
• Produce the current circulating strains, including the novel H1N1, as well as many historical A and B strains.
• Help clients search for common immunogenic epitopes.
• Provide high purity virus preparations using ultracentrifugation.
• Chemically inactivate influenza for immunological and PCR studies.

Our laboratories include BSL-2, BSL-2-enhanced and BSL-3. Clients can request traditional density gradient ultracentrifugation or chromatography-based purification. Other services we offer include production of viral components such as proteins and nucleic acid.

Influenza assays available at VIRAPUR

• Hemagglutination assay (HA) and Hemagglutination inhibition assay (HAI)
• TCID₅₀
• Egg Infectious Dose (EID₅₀)
• Plaque assay
• Microneutralization
• Fluorometric Neuraminidase assay (MUNANA)
• Drug screening
• ELISA

We are accustomed to providing services for preclinical testing labs, and can isolate and titrate virus from animal tissues and upper respiratory swabs and washes.

VIRAPUR's Quality System accommodates your development and regulatory needs. Projects are priced based on scientific and regulatory requirements.